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Determination of Hemoglobin by Cyanmethemoglobin metnod you can also watch the video of   ESTIMATION OF HEMOGLOBIN BY CYANMETHEMOGLOBIN  METHOD   https://youtu.be/CF10XpTtbnY Hemoglobin is a conjugated protein present inside the erythrocytes . Hemoglobin consist of  a prosthetic group named heam, which is  combined with protein called globin (Hemoglobin =  Heam + Globin). Other heam containing proteins in the body are Myoglobin, Cytochrome-C etc. Heam carries oxygen (O 2 ) from the lungs to the tissue cells and carbon dioxide (CO 2 ), the gaseous waste product from the cells to the lungs . After the normal life span of RBC (over 120 days), the red cells are destroyed by the reticuloendothelial cells (specially in the spleen) and the components of the hemoglobin undergo metabolic degeneration. Several methods are available for the estimation of hemoglobin in the blood. These are: Acid heamatin method (Sahli’s method) Cyanmethemoglobin method. Alkaliheamatin method. Haldane’

. . . . . . . . . . . . .. . . . . . . . . . . https://medicoinfo.org/hemoglobin-estimation-significance-procedure/ https://www.bioscience.com.pk/topics/cell-biology/item/167-cyanmethemoglobin-hemoglobin-cyanide-method-for-estimation-of-hemoglobin https://paramedicsworld.com/hematology-practicals/estimation-of-hemoglobin-by-sahlis-method-acid-hematin-method/medical-paramedical-studynotes

ESTIMATION OF HEMOGLOBIN BY SAHLI’s METHOD   a.k.a. ACID HEMATIN METHOD –  PRINCIPLE, PROCEDURE, NORMAL VALUES, CLINICAL SIGNIFICANCE you can also watch the video of   ESTIMATION OF HEMOGLOBIN BY SAHLI’s METHOD   https://youtu.be/orkqh56_BNU INTRODUCTION TO HEMOGLOBIN Hemoglobin (Hb or Hgb) is a red color pigment present in red blood cells (RBCs) comprises Fe2+ and Globin protein. It is Hemoglobin in RBCs that carries the oxygen from the lungs to the tissues and CO2 from body tissues to the lungs for excretion. Hemoglobin (Hb or Hgb) is responsible for the appearance of Red color RBCs and blood. Hemoglobin is a chromoprotein consisting of Globin molecule attached to 4 red colored Heme molecules. Hemoglobin synthesis requires the coordinated production of Heme and Globin. Heme is a prosthetic group that medicates reversible binding of oxygen by hemoglobin. Globin is the protein that surrounds and protects the Heme molecule. The Estimation of hemoglobin in the blood is c

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  Basic Laboratory Equipment Used in Microbiology | Biology In the laboratory, eight main types of instruments are used:-  1. Balance  2.  Centrifug e  3. Hot Air Oven  4. Incubators  5. Water Bath  6. Microscope 7. Autoclave  8. Laminar Flow. By advancement in technology to make the laboratory testings more accurate, fast, reliable and cost effective, we require these instruments with its proper care and maintenance. Equipment # 1. Balance : A balance is used to find out the mass of a substance by comparing it with known masses. These are used to weigh the chemicals accurately. Physical balance is based on the principle of moments according to which, when a body is in equilibrium, under the action of a number of forces acting on it in the same plane, the sum of the clockwise moments is equal to the sum of the anticlockwise moments. For a physical balance with both arms of equal lengths, Weight on left pan = Weight of standard masses in right pan. Since weight is constant at the place,