Cytochemical stainings, procedure and their significance in various haemopoietic disorders

Cytochemical stains are special stains used for staining peripheral blood and bone marrow smears that help in classifying and differentiating different types of leukaemias. They play a very important role in diagnosis, differentiation and classification of Leukemias.

 These stains can be
1) cytochemical stains ie they use cellular enzymatic reactions to impart staining
or
2) Immunocytochemical stains ie they identify cell specific antigens with the help of antibodies.
Not every lab is equipped with cytogenetics or immunophenotyping. And in that case cytochemical stains can be of immense help in identifying the lineage of the neoplasm.
Let us discuss the various cytochemical stains used in hematology especially in a low resource set up.
There are some Cytochemical stains, which is as follows:
  1. Leukocyte Alkaline Phosphatase (LAP)
  2. Myeloperoxidase Stain
  3. Sudan Black B 
  4. Acid Phosphatase (ACP)
  5. Esterases
  6. Periodic Acid-Schiff
  7. Toluidine Blue
  8. Perls Stain (Prussian Blue Reaction)

 

 

 

 

 

 

 FOR DETAILED INFO PLEASE OPEN THIS LINK:

 https://drive.google.com/file/d/1LT3JhvYX8-Y1_ltOZsRu5s-7KqAonbF0/view?usp=sharing

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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http://ilovepathology.com/cytochemical-stains-in-hematological-neoplasms/ 

 

https://www.jebmh.com/articles/cytochemical-stains-in-haematology.pdf.pdf 

 

 

 

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