PRACTICAL Determination of Bleeding Time by DUKE method. Duke method is easy to perform and requires minimal equipment or laboratory skill. INTRODUCTION A bleeding time test determines how quickly your blood clots to stop bleeding. The test is a basic assessment of how well your blood platelets work to form clots. Platelets are tiny cell fragments that circulate in your blood. They’re the first cells to react to a blood vessel injury. They seal off the wound to prevent more blood from escaping. Determination of bleeding time recognizes vascular defect and platelet disorder . REQUIREMENT - Alcohol sponge -Sterile lancet - Filter paper (circular) or blotting paper - Stopwatch or a watch with seconds hand. Note: Arrange all the necessary materials before starting the test. As the test is performed on the ...