Clearing of Tissue in Histopathology Lab

Clearing of Tissue in Histopathology Lab


 It’s Becoming Clearer…

The solvent used for this intermediate stage is usually xylene. The ‘clearing agent’ needs to be miscible with both ethanol and paraffin wax.

Following dehydration, the tissue is immersed in one to three different xylene immersions. In these stages, the ethanol is gradually replaced with xylene, and when the tissue is embedded, the xylene will be replaced by the molten paraffin wax.

Shrinkage of tissue can occur at these final stages as the xylene also removes fat residues left in the samples.




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