Medical/Science Study World

 Determination of Amylase It is Hydrolytic enzyme that splits complex carbohydrates Such as. starch and glycogen into simpler molecules of sugar e.g. Glucose. Normal Values According to the iodometric method of estimation normal values are: 60 - 180 somogyi units/dl 95 - 250 IU/dl Principle of Saccharometric Method by Somogyi Serum is incubated with a starch solution and the amount of reducing substance present is determined before and after the incubation. The difference gives a measure of amylolytic activity of serum. And Start s-t-a-r-c-h S t a r c h And N into .

Determination of Inorganic Phosphorus The name of the different methods used for inorganic Phosphorus determination is as following: - Chlorometric method - Gomorri method - Kuttner and Linchensteir method .

 Laboratory diagnosis of Haemophilia and von-willebrand disease Hemophilia is a bleeding disorder in which an individual’s blood does not clot properly. It arises due to problems or deficiencies with blood clotting proteins. Researchers estimate that around  1 in every 10,000 people Trusted Source  are born with hemophilia. The main diagnostic lab tests are clotting activity tests. Other tests can help diagnose hemophilia by ruling out other conditions or indicating that someone has an elevated risk of hemophilia. This article describes the main lab tests for hemophilia. It also discusses other hemophilia lab tests and explains how much they may cost. Blood tests The only way to definitively diagnose hemophilia is through blood tests to confirm low levels of clotting factors. Blood tests to diagnose hemophilia can be divided into two broad categories: screening tests, which are general assessments of blood health and clotting ability clotting factor tests, which specifically measure th

Principles of Metal impregnation Techniques Introduction Silver staining is a powerful technique for protein identification in gels as silver binds to chemical sidechains of the amino acids, including the carboxyl and sulfhydryl groups. It was introduced in 1972 and later adapted for protein separation from the polyacrylamide gel electrophoresis.  The nucleation sites in proteins promote the reduction of silver ions by formaldehyde into microscopic grains of elemental silver, enabling their detection. The treatment does not modify the tertiary structure of the proteins.  It has widespread applications owing to its high sensitivity, which typically is two orders of magnitude greater than the Coomassie and Ponceau staining methods and could even detect as little as 2 ng protein. There are two main protocols for silver staining: alkaline and acidic, depending on the silver impregnation. The alkaline method uses a diamine complex of silver nitrate with an alkaline environment (ammonia and

 Metachromatic staining    There are certain basic dyes belonging to aniline group that will differentiate particular tissue components by staining them a different color to that of original dye. The phenomenon is known as metachromasia. The tissue element reacting in this manner are said to be exhibiting metachromasia.  The generally accepted explanation of this phenomenon is that change in color is due to polymerization.  Sulfated substances are highly metachromatic e.g. Mast cell granules.    These contain Heparin which is highly sulfated.  Some of the common metachromatic dyes are :  -  Methylene blue -  Methyl violent -  Thionin  -  Crystal violent  -  Toluidine blue    Thionin and toluidine blue dyes are commonly used for quick staining of frozen selection using their metachromatic property to stain nucleus and cytoplasm differently.    Tissue components often demonstrated by metachromatic stains :  -  Amyloid Material :           Amyloid refers to the abnormal fibrous, extracell

 BMLS 601-18- Applied Hematology – II 1. Definition and classification of anaemias. https://drive.google.com/file/d/1n6Pz11u8C69-PkwntEtzkVVX9ZW-_Plw/view?usp=sharing https://www.slideshare.net/NabeelaBasha/rbc-68650939 2. Laboratory diagnosis of iron deficiency anaemia https://drive.google.com/file/d/1ARspw6ztFw_goHP9Q28ePLHzZtQT07Xe/view?usp=sharing 3. Laboratory diagnosis of megaloblastic anaemia 4. Laboratory diagnosis of haemolytic anaemia 5. Definition, classification and laboratory diagnosis of Leukemias Definition, Classification Laboratory diagnosis of Leukemia 6. Definition and laboratory diagnosis of Leukamoid Reactions 7. Cytochemical Staining's, Procedure and their significance in various Hemopoietic disorders. 8. Chromosomal studies in various Hematological disorders and their significance. 9. Mechanism of normal fibrinolysis and Laboratory diagnosis of hyperfibrinolysis. 10. Mechanism and laboratory diagnosis of disseminated intravascular coagulation (DIC). 11. Labor