Determination of Amylase

 Determination of Amylase

It is Hydrolytic enzyme that splits complex carbohydrates Such as. starch and glycogen into simpler molecules of sugar e.g. Glucose.



Normal Values


According to the iodometric method of estimation normal values are:

60 - 180 somogyi units/dl

95 - 250 IU/dl


Principle of Saccharometric Method by Somogyi

Serum is incubated with a starch solution and the amount of reducing substance present is determined before and after the incubation. The difference gives a measure of amylolytic activity of serum.





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