BASICS OF BIOCHEMISTRY BMLS 103-18

.

BMLS103-18- BASICS OF BIOCHEMISTRY

Theory

Aims /learning Objectives: The main objective of the subject is to impart the knowledge of apparatus, units, equipment's, volumetric analysis in the laboratory of clinical Biochemistry.

1. Introduction to Medical lab Technology. 

     (a) Role of Medical lab Technologist,

     (b) Ethics and responsibility, 

     (c) Safety measures

     (d) First aid.

https://iasst.gov.in/wp-content/uploads/2021/04/Labsafety_Manual_IASST_DS2.pdf



2. Cleaning and care of general laboratory glass ware and equipments. 

     (a) Steps involved in cleaning soda lime glass 

     (b) Steps involved in cleaning borosil glass.

     (c) Preparation of chromic acid solution. 

     (d) Storage.


Cleaning of Glassware

https://webpath.med.utah.edu/HISTHTML/MANUALS/ACIDGLAS.PDF

Preparation & Procedure of Chromic Acid


3. Distilled water. 

     (a) Method of preparation of distilled water 

     (b) Type of water distillation plants 

     (c) Storage of distilled water


4. Units of Measurement 

     (a) S.I unit and CGS units 

     (b) Conversion 

     (c) Strength, molecular weight, equivalent weight 

     (d)Normality, Molarity, Molality 

     (e) Numericals.

https://www.slideshare.net/slideshow/si-units-38495432/38495432

https://www.ntschools.org/cms/lib/NY19000908/Centricity/Domain/114/Chapter%202%20Measurement%20and%20Calculations.ppt


5. Calibration of volumetric apparatus 

     (a) Flask 

     (b) Pipettes 

     (c) Burettes 

     (d) Cylinders

https://chem.libretexts.org/Courses/University_of_California_Davis/Chem_4A_Lab%3A_General_Chemistry_for_Majors_I/Chem_4A%3A_Laboratory_Manual/04_1%3A_Calibration_of_Volumetric_Glassware_(Experiment)

https://lab-training.com/volumetric-apparatus-use-calibration/


6. Analytical balance 

     (a) Principle 

     (b) Working 

     (c) Maintenance

https://microbenotes.com/analytical-balance-principle-parts-types-examples/

https://www.ossila.com/pages/maintenance-tips-for-analytical-balances-and-microbalances


7. Concept of pH 

(a) Definition 

(b) Henderson Hasselbatch equation 

(c) Pka value 

(d) pH indicator

(e) Methods of measurement of pH 

(i) pH paper 

(ii) pH meter: Principle, working, maintenance and calibration of pH meter


8. Volumetric analysis 

(a) Normal and molar solutions 

(b) Standard solutions 

(c) Preparation of reagents 

(d) Storage of chemicals


https://labpedia.net/solutions-part-2-preparation-of-solutions-molar-normal-dilution/

https://pharmabeginers.com/general-reagent-solution-preparation/

https://ehs.wisc.edu/labs-research/chemical-safety/chemical-safety-guide/chemical-storage/

https://protect.iu.edu/environmental-health/research-safety/lab-chemicals.html

https://www.lehman.edu/administration/environmental-health-safety/documents/chemical-storage.pdf

 

.

.

Comments

Post a Comment

Popular posts from this blog

Applied Hematology–II (BMLS 601-18)

 BMLS 601-18- Applied Hematology – II 1. Definition and classification of anaemias. https://drive.google.com/file/d/1n6Pz11u8C69-PkwntEtzkVVX9ZW-_Plw/view?usp=sharing https://www.slideshare.net/NabeelaBasha/rbc-68650939 2. Laboratory diagnosis of iron deficiency anaemia https://drive.google.com/file/d/1ARspw6ztFw_goHP9Q28ePLHzZtQT07Xe/view?usp=sharing 3. Laboratory diagnosis of megaloblastic anaemia 4. Laboratory diagnosis of haemolytic anaemia 5. Definition, classification and laboratory diagnosis of Leukemias Definition, Classification Laboratory diagnosis of Leukemia 6. Definition and laboratory diagnosis of Leukamoid Reactions 7. Cytochemical Staining's, Procedure and their significance in various Hemopoietic disorders. 8. Chromosomal studies in various Hematological disorders and their significance. 9. Mechanism of normal fibrinolysis and Laboratory diagnosis of hyperfibrinolysis. 10. Mechanism and laboratory diagnosis of disseminated intravascular coagulation (DIC). 11. Labor...
Read more

HISTOTECHNOLOGY – II & Cytology BMLS 605-18

HISTOTECHNOLOGY – II & Cytology  BMLS 605-18 Theory Aims and Objectives: The students will learn about various staining procedures for demonstration of different substances & various cytological investigations. The students will learn about special staining procedures & handling & testing of various cytological specimens. HISTOPATHOLOGY https://rajswasthya.nic.in/RHSDP%20Training%20Modules/Lab.%20Tech/Histo/Introduction.pdf STAINING https://rajswasthya.nic.in/RHSDP%20Training%20Modules/Lab.%20Tech/Histo/Chapter%206.pdf HISTOCHEMISTRY https://www.du.edu.eg/upFilesCenter/exStore/sci/1584370154.pdf 1. Cryostat sectioning, its applications in diagnostic histopathology. https://drive.google.com/file/d/1BPHfo5n_LoDnUWCOW0ZzGEF45jo9fWps/view?usp=sharing https://www.slideshare.net/Mahmoud19905/frozen-section-and-cryostat https://www.pathologyoutlines.com/topic/methodsfrozen.html 2. Special Staining Procedures for detection of a. Connective tissue elements, Trichrome stainin...
Read more

BMLS 501-18 Applied Hematology-I NOTES

 BMLS 501-18 Applied Hematology-I 1. Quality assurance in haematology. a. Internal and external quality control including reference preparation b. Routine quality assurance protocol c. Statistical analysis i.e. Standard deviation, Co-efficient variation, accuracy and precision https://medstudyworld.blogspot.com/2021/08/quality-assurance.html https://drive.google.com/file/d/1Fzba-s9jKUoOPSz1zHLteZ532EdgYiwO/view?usp=sharing 2. Safety precautions in haematology https://medstudyworld.blogspot.com/2021/08/safety-precautions-in-hematology-lab.html https://doctorlib.info/hematology/rodak-hematology-clinical-principles-applications/3.html https://www.slideshare.net/NegashRealis/safety-and-infection-control-in-hematology-laboratory 3. Basic concepts of automation in haemotology with special reference to: a. Blood cell counter b. Coagulometer. https://medstudyworld.blogspot.com/2021/08/automation-in-blood-cell-counter.html 4. Bone marrow examination https://drive.google.com/file/d/1oEwnXCkU...
Read more