BASICS OF BIOCHEMISTRY BMLS 103-18

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BMLS103-18- BASICS OF BIOCHEMISTRY

Theory

Aims /learning Objectives: The main objective of the subject is to impart the knowledge of apparatus, units, equipment's, volumetric analysis in the laboratory of clinical Biochemistry.

1. Introduction to Medical lab Technology. 

     (a) Role of Medical lab Technologist,

     (b) Ethics and responsibility, 

     (c) Safety measures

     (d) First aid.

https://iasst.gov.in/wp-content/uploads/2021/04/Labsafety_Manual_IASST_DS2.pdf



2. Cleaning and care of general laboratory glass ware and equipments. 

     (a) Steps involved in cleaning soda lime glass 

     (b) Steps involved in cleaning borosil glass.

     (c) Preparation of chromic acid solution. 

     (d) Storage.


Cleaning of Glassware

https://webpath.med.utah.edu/HISTHTML/MANUALS/ACIDGLAS.PDF

Preparation & Procedure of Chromic Acid


3. Distilled water. 

     (a) Method of preparation of distilled water 

     (b) Type of water distillation plants 

     (c) Storage of distilled water


4. Units of Measurement 

     (a) S.I unit and CGS units 

     (b) Conversion 

     (c) Strength, molecular weight, equivalent weight 

     (d)Normality, Molarity, Molality 

     (e) Numericals.

https://www.slideshare.net/slideshow/si-units-38495432/38495432

https://www.ntschools.org/cms/lib/NY19000908/Centricity/Domain/114/Chapter%202%20Measurement%20and%20Calculations.ppt


5. Calibration of volumetric apparatus 

     (a) Flask 

     (b) Pipettes 

     (c) Burettes 

     (d) Cylinders

https://chem.libretexts.org/Courses/University_of_California_Davis/Chem_4A_Lab%3A_General_Chemistry_for_Majors_I/Chem_4A%3A_Laboratory_Manual/04_1%3A_Calibration_of_Volumetric_Glassware_(Experiment)

https://lab-training.com/volumetric-apparatus-use-calibration/


6. Analytical balance 

     (a) Principle 

     (b) Working 

     (c) Maintenance

https://microbenotes.com/analytical-balance-principle-parts-types-examples/

https://www.ossila.com/pages/maintenance-tips-for-analytical-balances-and-microbalances


7. Concept of pH 

(a) Definition 

(b) Henderson Hasselbatch equation 

(c) Pka value 

(d) pH indicator

(e) Methods of measurement of pH 

(i) pH paper 

(ii) pH meter 

(iii)Principle, working, maintenance and calibration of pH meter


8. Volumetric analysis 

(a) Normal and molar solutions 

(b) Standard solutions 

(c) Preparation of reagents 

(d)Storage of chemicals




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