Applied Bacteriology BMLS 307-18

Applied Bacteriology

BMLS 307-18

THEORY


 1. Antibiotic susceptibility testing in bacteriology 

a. Definition of antibiotics 

b. Culture medium used for Antibiotic susceptibility testing 

c. Preparation and standardization of inoculum 

https://www.slideshare.net/slideshow/antibiotic-sensitivity-tests/40161695

d. Control bacterial strains 

https://www.open.edu/openlearncreate/mod/oucontent/view.php?id=172394&section=6.3#:~:text=Control%20strains%20are%20bacterial%20isolates,well%20characterised%20antibiotic%20susceptibility%20profile.


e. Choice of antibiotics 

f. MIC and MBC : Concepts and methods for determination 

https://www.qlaboratories.com/minimum-inhibitory-mic-and-minimum-bactericidal-concentration-mbc-evaluations-as-rd-tools/

https://www.nelsonlabs.com/testing/mic-mbc/#:~:text=Minimum%20Inhibitory%20Concentration%20(MIC)%20and,20%2Dhour%20period%20of%20incubated

g. Various methods of Antibiotic susceptibility testing with special reference to Kirby-Bauer method and Stokes method.

https://microbenotes.com/kirby-bauer-disc-diffusion/

https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_I/09%3A_Kirby-Bauer_(Antibiotic_Sensitivity)

https://universe84a.com/collection/stokes/

https://biologynotesonline.com/stokes-disc-diffusion-method-principle-procedure-result/#google_vignette


h. Tests for production of β-lactamase.

https://microbenotes.com/beta-%CE%B2-lactamase-test-objectives-principle-procedure-and-results/


2. Bacteriological examination of air

a. Examination of Air a) Significance of air bacteriology in healthcare facilities b) Settle plate method c) Types of air sampling instruments d) Collection processing and reporting of an air sample

https://www.slideshare.net/slideshow/air-bacteriology/234910584

https://www.brainkart.com/article/Bacteriology-of-Air_18628/#google_vignette


4. Sterility testing of I/v fluids a. Collection, transportation and processing of I/v fluids for bacterial contamination b. Recording the result and interpretation

https://www.slideshare.net/slideshow/sterility-test-86299788/86299788

https://www.npra.gov.my/images/Announcement/Archives/Slides-amv/AMV%20-%20STERILITY%20TEST.pdf

5. Nosocomial Infection: a) Bacteriological surveillance of hospital environment. b) Role of microbiology laboratory in control of nosocomial infections

https://www.slideshare.net/slideshow/nosocomial-infection/3659542

https://www.slideshare.net/slideshow/nosocomial-infection-control/29540605


6. Preservation methods for microbes

a. Basic concepts of preservation of microbes b. Why do we need to preserve bacteria c. Principle and procedures of various preservation methods with special reference to lyophilization.

https://www.slideshare.net/slideshow/preservation-of-microbes/249294918

https://www.slideshare.net/slideshow/microbial-culture-preservation-and-its-methods/251078938


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