Histopathology

 



1. Introduction and definition:

1.1 Histology

1.2 Histopathology

1.3 Biopsy

1.4 Autopsy

1.5 Autolysis

1.6 Putrefaction

1.7 Fixation

1.8 Deparaffinization

1.9 Blueing

              Chapter 1 Notes


Preparation of Tissue 

2.1 Unfixed Tissue preparations: Imprint methods Impression Smears, Teased

preparation, Squashed preparation, Frozen section.

2.2 Fixed Tissue Preparations: Paraffin embedding, Celloidin embedding, Gelatin

embedding.

              Chapter 2 Notes

 

3. Reception of Specimen

3.1 Reception, recording, labelling and preservation of histological specimen

            Chapter 3


4. Fixation (Histological Specimens)

4.1 Introduction

4.2 Classification & Composition of various fixatives

4.3 Advantages/Disadvantages of various fixatives

             Fixation


5. Decalcification

Introduction

Principle & Procedure of Acid Method of Decalcification

https://docs.google.com/document/d/e/2PACX-1vTZmgHTO3Xn2S8wUikzIsSigUbLOVyEkn6FkgUYMJvY9KFidX8QSIBVEZuDhug0EQapC2oZMhg4Hmo8/pub


6. Processing (by Paraffin Technique) 

6.1 Dehydration

6.2 Clearing

6.3 Infiltration and Impregnation

6.4 Paraffin embedding


7. Microtomy (4 hrs)

7.1 Microtome & Microtome Knives

7.1.1 Types of Microtome

7.1.2 Principle and Working of Rotary Microtome & Freezing Microtome

7.1.3 Types of Microtome knives

7.1.4 Sharpening of knives (Honing & Stropping technique)

7.1.5 Care and Maintenance of Microtome & its Knives

8. Section Cutting (3 hrs)

8.1 Process of section cutting

8.2 Errors /cutting faults in sections and their remedies

9. Frozen section technique (3 hrs)

9.1 Process of Frozen section Technique

9.2 Principle, Procedure & Interpretation of H&E, Polychrome Methylene Blue

10. Routine & Special Stains (4 hrs)

10.1 Principle, Procedure & Interpretation of Routine Stain: Haematoxylin and

Eosin

10.2 Principle, Procedure & Interpretation of Special Stains:

PAS

SILVER IMPREGNATION

OIL RED O

MASSON TRICHOME

11. Automation (1 hr)

11.1 Histokinete (Automatic tissue processor): Use, Care, &









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