AEC (Absolute Eosinophil Count)

 

Alternative Names

Eosinophils; Absolute eosinophil count

Eosinophils are white blood cells of the body which play an important role in protecting the immunity of the body. They are stored in the bone marrow and can survive up to few weeks. An absolute eosinophil count is a blood test that measures the number of one type of white blood cells called eosinophils. Eosinophils become active when you have certain allergic diseases, infections, and other medical conditions. The measure of the total quantity of eosinophil in the blood is called as total eosinophil count. The increase in eosinophils is called as eosinophilia and happens in certain disease conditions. 

Principle

Blood is diluted with a special diluting fluid, which removes red cells and stains the eosinophils red. These cells are then counted under low power (10X) in a known volume of fluid by using Neubauer counting chamber.

 

Requirements

  1   Microscope

  2   Improved Neubauer Chamber or Fuch-Rosenthal counting chamber

  3   Diluting fluid: (Hingleman's solution):  it is prepared as follows:

        a)  Yellow eosin  : 0.5 g

        b)  95% phenol  : 0.5 ml

        c)  Formalin : 0.5 ml

        d)  Distilled water : 99 ml

 

Normal range :  40-440/cu mm (μl

 

Specimen:  EDTA or heparinized blood

 

Procedure

Pipette 0.36 ml of diluting fluid in a test tube.

Add 0.04 ml of blood (use Hb pipette, twice).

Mix and keep for 10 minutes.

Mix the diluent and charge the counting chamber,

Let it stand under a moist petri dish for about 2 to 3 minutes.

Count the cells (eosinophils) under low power objective lens  with reduced light.

-  If improved Neubauer counting chamber is used, count cells in all nine squares (In the case of total leucocyte count  only four squares at the corner are used).

 

Calculations

Total number of eosinophils, cu mm (µl)

 

=     Number of cells counted × 10

     _______________________________

                            0.9

Note:

a) Dilution = 10

b) Volume of fluid   =  area counted  x  depth

                                 =   9 sq. mm    x      0.1

                                 =    0.9

Clinical Significance

Increased eosinophil count is often associated with allergic reactions, parasitic infections, brucellosis and in certain leukemias. Increase in the adrenal function (hyperadrenalism or Cushing's syndrome) is associated with a fall in eosinophil count.

 

 

 

 

 

 

 

 

 

 

 

 https://labpedia.net/eosinophil-count-absolute-eosinophil-count/

 https://www.myupchar.com/en/test/absolute-eosinophil-count-test

 http://biolabdiagnostics.com/image/catalog/pdf/EOSINOPHIL%20Fluid%20KI.pdf

 https://www.lybrate.com/lab-test/absolute-eosinophil-count

 https://labmonk.com/determination-eosinophil-count

 https://www.medikaynak.com/en/medicalculation/absolute-eosinophil-count-calculator

 

 .

.

.

.

.

Comments

Post a Comment

Popular posts from this blog

BMLS 501-18 Applied Hematology-I NOTES

 BMLS 501-18 Applied Hematology-I 1. Quality assurance in haematology. a. Internal and external quality control including reference preparation b. Routine quality assurance protocol c. Statistical analysis i.e. Standard deviation, Co-efficient variation, accuracy and precision https://medstudyworld.blogspot.com/2021/08/quality-assurance.html https://drive.google.com/file/d/1Fzba-s9jKUoOPSz1zHLteZ532EdgYiwO/view?usp=sharing 2. Safety precautions in haematology https://medstudyworld.blogspot.com/2021/08/safety-precautions-in-hematology-lab.html https://doctorlib.info/hematology/rodak-hematology-clinical-principles-applications/3.html https://www.slideshare.net/NegashRealis/safety-and-infection-control-in-hematology-laboratory 3. Basic concepts of automation in haemotology with special reference to: a. Blood cell counter b. Coagulometer. https://medstudyworld.blogspot.com/2021/08/automation-in-blood-cell-counter.html 4. Bone marrow examination https://drive.google.com/file/d/1oEwnXCkU...
Read more

BASICS OF BIOCHEMISTRY BMLS 103-18

. BMLS103-18- BASICS OF BIOCHEMISTRY Theory Aims /learning Objectives: The main objective of the subject is to impart the knowledge of apparatus, units, equipment's, volumetric analysis in the laboratory of clinical Biochemistry. 1. Introduction to Medical lab Technology.       (a) Role of Medical lab Technologist,      (b) Ethics and responsibility,       (c) Safety measures      (d) First aid . https://iasst.gov.in/wp-content/uploads/2021/04/Labsafety_Manual_IASST_DS2.pdf 2. Cleaning and care of general laboratory glass ware and equipments.       (a) Steps involved in cleaning soda lime glass        (b) Steps involved in cleaning borosil glass.      (c) Preparation of chromic acid solution .       (d) Sto rage. Cleaning of Glassware https://webpath.med.utah.edu/HISTHTML/MANUALS/ACIDGLAS.PDF Preparation & Procedure of Chromic Acid 3. Disti...
Read more

B.Sc. MLS 5th Sem Histotechnology Notes (BMLS 504-18)

 B.Sc. MLS 5th Sem Histotechnology Notes 1. Introduction to histotechnology 2. Care and maintenance of laboratory equipment used in histotechnology 3. Safety measures in a histopathology laboratory 4. Basic concepts about routine methods of examination of tissues 5. Collection and transportation of specimens for histological examination 6. Basic concepts of fixation 7. Various types of fixatives used in a routine histopathology laboratory:        i. Simple fixatives        ii. Compound fixatives,        iii. Special fixatives for demonstration of various tissue elements 8. Decalcification :         a. Criteria of a good decalcification agent,         b. Technique of decalcification followed with selection of tissue, fixation, decalcification,            neutralization of acid and thorough washing.         c. Various ty...
Read more